Composite
Part:BBa_K1898150
Designed by: Fiona Tsai Group: iGEM16_TAS_Taipei (2016-10-10)
Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator
BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Synthesis Report
This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:
- Prefix and suffix are highlighted in orange
- BBa_K880005 is highlighted in red
- CH5H is highlighted in light blue
- 10x Histidine is highlighted in green
- B0015 is highlighted in dark blue
IDT report shows that the sequence is successfully synthesized:
[edit]
Categories
Parameters
//awards/part_collection/2016
None |